This test is essential for diagnosing infections like spontaneous bacterial peritonitis (SBP), which can be life-threatening if left untreated.
The C/S Ascitic Fluid Test, also known as Culture and Sensitivity Test for Ascitic Fluid, is a critical diagnostic procedure used to analyze ascitic fluid, which is the fluid that accumulates in the peritoneal cavity (the space around the abdominal organs). This test helps identify infections and assess the cause of ascites, a condition characterized by the buildup of fluid in the abdomen. Ascites is a common complication of liver disease, such as cirrhosis, but it can also be caused by cancers, heart failure, tuberculosis, and other medical conditions.
The Culture and Sensitivity (C/S) aspect of the test involves both culturing the ascitic fluid to identify any bacterial, viral, or fungal infections, and testing the sensitivity of the microorganisms to different antibiotics to determine the most effective treatment options.
1] Preparation: The patient is positioned comfortably, often sitting or lying down. The area where the needle will be inserted is cleaned and sterilized to prevent infection.
2] Paracentesis: A sterile needle or catheter is inserted into the abdomen, typically below the belly button, to collect the ascitic fluid. The procedure is usually performed under local anesthesia to minimize discomfort. In some cases, ultrasound guidance may be used to ensure accurate needle placement.
3] Fluid Collection: A small amount of ascitic fluid is drawn into sterile containers. Multiple samples may be taken, especially if the test is being used to identify multiple conditions (e.g., infection, cancer cells, or other abnormalities).
4] Laboratory Analysis: The collected fluid is sent to the laboratory, where it undergoes microbiological analysis (culture), and tests for the presence of white blood cells, proteins, glucose, and other biochemical markers. The culture process involves growing any microorganisms present in the fluid to identify infections.
5] Sensitivity Testing: If bacteria are detected, the culture is tested for antibiotic sensitivity to determine which antibiotics would be most effective in treating the infection.
1] Culture Results:
A] Positive Culture: If bacteria, fungi, or other pathogens grow from the fluid, this suggests an infection, such as spontaneous bacterial peritonitis or other infections related to liver cirrhosis or cancer.
B] Negative Culture: A negative culture may suggest the absence of infection, but further tests may be needed to investigate other causes of ascites, such as malignancy or tuberculosis.
2] White Blood Cell Count: Elevated levels of white blood cells in the ascitic fluid can indicate infection or inflammation.
3] Albumin and Protein Levels: Low albumin and protein levels in the ascitic fluid are often associated with liver cirrhosis and portal hypertension. High protein levels may suggest malignancy or tuberculosis.
4] Glucose Levels: Low glucose levels in the ascitic fluid may point to infection, while normal glucose levels are typically seen in conditions like heart failure.
5] Cancer Cells: The presence of cancer cells indicates that the ascites may be due to cancer, especially metastatic cancer.
Choosing pathology lab test in Pune for your C/S Ascitic Fluid Test ensures you receive accurate, reliable results with personalized care. We prioritize high-quality diagnostics and use advanced laboratory technologies to ensure that your ascitic fluid analysis is precise and comprehensive. Our expert team of healthcare professionals is dedicated to helping you understand your results and guiding you through the next steps in your treatment. Whether you're undergoing tests for liver disease, infection, or cancer, Diagnopein provides a compassionate and efficient service with quick turnaround times. Additionally, we offer home sample collection for your convenience, making it easier for you to get tested without needing to leave home. With our affordable pricing, excellent customer service, and commitment to quality, Diagnopein is your trusted partner in managing and diagnosing ascitic fluid-related conditions.
1. Culture Method
2. Sample
3. Colony Count
4. Organism(s) Isolated
5. Culture Report: Culture yields growth of
6. Culture isolated after 7 days :
7. Culture isolated after 14 days:
8. Culture isolated after 21 days:
9. Ampicillin
10. Amikacin
11. Amoxicillin clavulanate
12. cefoperazon+sulbactam
13. Cefuroxime
14. Cefepime
15. Cefotaxime
16. Ciprofloxacin
17. Ertapenem
18. Gentamicin
19. Imipenem
20. Meropenem
21. Norfloxacin
22. Nitrofurantoin
23. Piperacillin-tazobactam
24. Trimethoprim-Sulfamethoxazole (Cotrimoxazole)
No special preparation is required for the test, but you may be asked to avoid eating or drinking for a short period before the paracentesis procedure.
The test is recommended if you have ascites, especially with symptoms like abdominal pain, fever, or signs of infection, or if you have underlying liver disease, cancer, or other conditions that could cause ascites.
A healthcare provider collects a sample of ascitic fluid through a procedure called paracentesis, where a needle is inserted into the abdomen to withdraw the fluid for analysis in a lab.
It helps diagnose infections, assess the cause of ascites, guide appropriate treatment (including antibiotic therapy), and monitor conditions like liver cirrhosis and cancer.