The Sputum for AFB (Acid-Fast Bacilli) test is a critical diagnostic tool for detecting tuberculosis (TB) and other mycobacterial infections.
This test, often performed using the Ziehl-Neelsen (ZN) staining method, is a reliable and cost-effective technique for identifying acid-fast bacteria in a patient’s sputum. If you are looking for a pathology lab near me in Pune to conduct this test, it is important to choose a trusted and well-equipped laboratory for accurate results.
The Sputum for AFB (ZN Stain) test involves examining a sample of sputum under a microscope after treating it with special dyes. The Ziehl-Neelsen stain is used to highlight acid-fast bacilli, such as Mycobacterium tuberculosis, which are the causative agents of TB. Early and accurate detection of TB through this test is vital for timely treatment and prevention of further transmission.
Doctors typically recommend the Sputum for AFB (ZN Stain) test if a patient exhibits symptoms suggestive of TB, including:
1] Persistent cough lasting more than two weeks
2] Unexplained weight loss
3] Night sweats and fever
4] Blood-streaked sputum
The test is also performed for individuals who are immunocompromised, such as HIV-positive patients, as they are at a higher risk of developing TB.
For accurate results, it’s essential to visit a reliable pathology lab near me in Pune. The lab should have trained technicians and advanced microscopy facilities to perform the ZN staining method effectively. Many reputed pathology labs in Pune offer online booking options, home sample collection, and quick pathology lab test results, making the process convenient for patients.
1] Sample Collection: The patient is instructed to provide an early morning sputum sample. If sputum is not easily produced, a saline nebulizer may be used to stimulate expectoration.
2] Staining Process: The sample is spread on a slide, air-dried, and fixed with heat. It is then treated with the Ziehl-Neelsen stain, which includes carbol fuchsin, acid-alcohol, and methylene blue.
3] Microscopic Examination: A skilled pathologist examines the stained slide under a microscope to identify the presence of acid-fast bacilli.
The results of the Sputum for AFB (ZN Stain) test are typically reported as:
A] Positive: Acid-fast bacilli are detected, indicating a TB infection.
B] Negative: No acid-fast bacilli are found. In such cases, additional tests like GeneXpert or culture may be recommended for confirmation.
Early diagnosis of TB through the Sputum for AFB (ZN Stain) test helps in:
A] Initiating prompt treatment to prevent complications
B] Reducing the spread of TB to others
C] Monitoring the effectiveness of ongoing therapy
Diagnopein stands out as the best diagnostic centre in Pune, offering unparalleled accuracy and reliability in diagnostic services. As an NABL accredited lab, Diagnopein adheres to the highest standards of quality and precision, ensuring that every test, including the Sputum for AFB (ZN Stain) test, is conducted with utmost care and expertise. The laboratory is equipped with advanced technology and staffed by highly trained professionals, guaranteeing accurate reports that you can trust. With a commitment to delivering timely results and exceptional patient care, Diagnopein is the preferred choice for individuals seeking dependable diagnostic solutions in Pune.
1. Method
2. SPECIMEN
3. Space
4. Ziehl Neelsen Stain
Generally, no special preparation is required. However, you may be advised to avoid eating or drinking for a certain period before the test to ensure a clear sample.
The test is designed to detect Mycobacterium tuberculosis in sputum samples, helping diagnose active tuberculosis and monitor treatment effectiveness.
At Diagnopein, we strive to provide results within 24-48 hours. Our team will inform you of your results and any necessary follow-up steps promptly.
A sputum sample is collected, typically early in the morning, and sent to the laboratory for staining using the Ziehl-Neelsen method, which identifies acid-fast bacilli.
